Question 1

A member of the Enterobacteriaceae is non-motile. The other biochemical reactions do not correlate with the identification of Klebsiella or Shigella species. What is the next step in the workup of this organism?

Accepted Answer

A)   Repeat the biochemical tests
B)   Perform serotyping
C)   Repeat the motility test at 25°C
D)   Perform an oxidase testE) A) and B)
F) A) and C)

Question 2

A day shift microbiologist encounters an oxidase negative, non-lactose fermenter in a urine culture. To determine if it is a member of the Enterobacteriaceae or a nonfermenter, he sets up a TSI agar slant. After 10 hours of incubation at 35°C in ambient air, the evening shift microbiologist reads the tube as a yellow slant and yellow deep or butt. This result

Accepted Answer

A)   indicates a member of the Enterobacteriaceae.
B)   indicates a nonfermenter.
C)   could be due to a gram-positive organism. Perform a Gram stain.
D)   is invalid.E) B) and C)
F) A) and C)

Question 3

Prior to adding reagent(s)  and interpretation, the conventional methyl red test requires incubation

Accepted Answer

A)   at 42°C.
B)   for 24 hours.
C)   at 25°C.
D)   for 48 hours.E) B) and D)
F) A) and B)

Question 4

An oxidase-negative, gram-negative rod turns the urea agar slant pink within 4 hours. This indicates

Accepted Answer

A)   an invalid reaction.
B)   a possible Proteus species.
C)   that the organism will probably also be VP positive.
D)   that it will be susceptible to vancomycin.E) All of the above
F) C) and D)

Question 5

The end result of a positive enzyme immunoassay is read as

Accepted Answer

A)   agglutination.
B)   fluorescence.
C)   a lack of color.
D)   a colored compound.E) B) and D)
F) All of the above

Question 6

The detection of acetyl-methyl carbinol or acetoin is the basis of the _________ test.

Accepted Answer

A)   Voges-Proskauer
B)   methyl red
C)   IMVIC
D)   citrateE) C) and D)
F) A) and B)

Question 7

Which biochemical test requires the use of 10% ferric chloride to detect a positive reaction?

Accepted Answer

A)   Tryptophan deaminase
B)   Phenylalanine deaminase
C)   Deamination of lysine
D)   Both a and b
E)   Both b and cF) None of the above
G) B) and E)

Question 8

A positive citrate test is due to the organism's ability to

Accepted Answer

A)   break down tryptophan.
B)   use citrate as the sole source of carbon.
C)   extract nitrogen from ammonium salt.
D)   both b and c.
E)   all of the above.F) A) and B)
G) B) and C) D

Question 9

The proper amount and order of addition of reagent(s)  for the test for the production of acetoin is

Accepted Answer

A)   0.6 mL of 5.0% alpha naphthol and 0.2 mL of 40% KOH.
B)   0.6 mL of 40% KOH and 0.2 mL of 5.0% alpha-naphthol.
C)   0.2 mL of 5.0% alpha naphthol and 0.6 mL of 40% KOH.
D)   0.2 mL of 5.0% 40% KOH and 0.6 mL of 5.0% alpha naphthol.E) None of the above
F) B) and D)

Question 10

The metabolism of carbohydrates by Pseudomonas aeruginosa is determined using

Accepted Answer

A)   the ONPG test.
B)   a phenol red carbohydrate broth with a Durham tube.
C)   duplicate semisolid carbohydrate tubes with and without oil.
D)   TSI agar.E) B) and D)
F) B) and C)

Question 11

A microbiologist sets up a set of decarboxylase tubes for identification of a glucose fermenting, gram-negative rod. After 24 hours, the tubes reveal
Base control: gray
Arginine: purple
Lysine: yellow
Ornithine: purple
These tubes should be interpreted as

Accepted Answer

A)   all positive.
B)   invalid.
C)   positive for arginine and ornithine.
D)   positive for lysine.E) A) and C)
F) All of the above

Question 12

A yellow ONPG test indicates production of

Accepted Answer

A)   beta-galactosidase.
B)   beta-galactoside permease.
C)   lactase.
D)   both a and b.E) A) and B)
F) C) and D) A

Question 13

Which of the following reagents are required for testing of nitrate reduction?
1)  Alpha-naphthylamine
2)  Sulfanilic acid
3)  Zinc dust
4)  Alpha-naphthol
5)  40% KOH

Accepted Answer

A)   1 and 2
B)   1, 2, and 3
C)   4 and 5
D)   3, 4 and 5E) A) and D)
F) A) and C)

Question 14

The cytochrome oxidase test is used primarily for the identification of

Accepted Answer

A)   gram-positive organisms.
B)   gram-negative organisms.
C)   Veillonella species.
D)   Micrococcus species.E) B) and C)
F) A) and C)

Question 15

Which procedure is best suited for determining the HIV viral load of an individual?

Accepted Answer

A)   Direct fluorescent antibody assay
B)   Monoclonal antibodies
C)   Real-time polymerase chain reaction
D)   DNA fingerprintingE) All of the above
F) A) and C)

Question 16

One potential disadvantage of an automated identification system is

Accepted Answer

A)   the need for extensive training.
B)   the cost.
C)   a lack of specificity.
D)   a lack of rapid turnaround.E) B) and C)
F) A) and B) B

Question 17

A non-motile, VP-positive, gram-negative rod is a possible

Accepted Answer

A)   Shigella species.
B)   Enterobacter species.
C)   Serratia species.
D)   Klebsiella species.E) All of the above
F) C) and D)

Question 18

In nucleic acid synthesis, which enzyme is responsible for replication of DNA?

Accepted Answer

A)   Restriction endonucleases
B)   DNA ligase
C)   Isomerase
D)   DNA polymeraseE) A) and B)
F) A) and D)

Question 19

Phenol red carbohydrate broth should be incubated at/in ___________________
1)  -25°C.
2)  35°C.
3)  ambient air.
4)  CO2.

Accepted Answer

A)   1 and 3
B)   1 and 4
C)   2 and 3
D)   2 and 4E) A) and B)
F) None of the above

Question 20

After the addition of reagents for the nitrate test, the tube remains colorless. To determine if the organism reduced nitrates to nitrogen gas, you must now add

Accepted Answer

A)   10% ferric chloride.
B)   zinc dust.
C)   TTC.
D)   nothing-the test is complete.E) A) and B)
F) A) and C)

Exam 9: Final Identification

A member of the Enterobacteriaceae is non-motile. The other biochemical reactions do not correlate with the identification of Klebsiella or Shigella species. What is the next step in the workup of this organism?

Correct Answer
verified

Correct Answer
verified

Prior to adding reagent(s) and interpretation, the conventional methyl red test requires incubation

Correct Answer
verified

An oxidase-negative, gram-negative rod turns the urea agar slant pink within 4 hours. This indicates

Correct Answer
verified

The end result of a positive enzyme immunoassay is read as

Correct Answer
verified

The detection of acetyl-methyl carbinol or acetoin is the basis of the _________ test.

Correct Answer
verified

Which biochemical test requires the use of 10% ferric chloride to detect a positive reaction?

Correct Answer
verified

A positive citrate test is due to the organism's ability to

Correct Answer
verified
D

The proper amount and order of addition of reagent(s) for the test for the production of acetoin is

Correct Answer
verified

The metabolism of carbohydrates by Pseudomonas aeruginosa is determined using

Correct Answer
verified

A microbiologist sets up a set of decarboxylase tubes for identification of a glucose fermenting, gram-negative rod. After 24 hours, the tubes reveal Base control: gray Arginine: purple Lysine: yellow Ornithine: purple These tubes should be interpreted as

Correct Answer
verified

A yellow ONPG test indicates production of

Correct Answer
verified
A

Which of the following reagents are required for testing of nitrate reduction? 1) Alpha-naphthylamine 2) Sulfanilic acid 3) Zinc dust 4) Alpha-naphthol 5) 40% KOH

Correct Answer
verified

The cytochrome oxidase test is used primarily for the identification of

Correct Answer
verified

Which procedure is best suited for determining the HIV viral load of an individual?

Correct Answer
verified

One potential disadvantage of an automated identification system is

Correct Answer
verified
B

A non-motile, VP-positive, gram-negative rod is a possible

Correct Answer
verified

In nucleic acid synthesis, which enzyme is responsible for replication of DNA?

Correct Answer
verified

Phenol red carbohydrate broth should be incubated at/in ___________________ 1) -25°C. 2) 35°C. 3) ambient air. 4) CO2.

Correct Answer
verified

After the addition of reagents for the nitrate test, the tube remains colorless. To determine if the organism reduced nitrates to nitrogen gas, you must now add

Correct Answer
verified

Exam 9: Final Identification

A member of the Enterobacteriaceae is non-motile. The other biochemical reactions do not correlate with the identification of Klebsiella or Shigella species. What is the next step in the workup of this organism?

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

Prior to adding reagent(s) and interpretation, the conventional methyl red test requires incubation

Correct AnswerverifiedShow Answer

An oxidase-negative, gram-negative rod turns the urea agar slant pink within 4 hours. This indicates

Correct AnswerverifiedShow Answer

The end result of a positive enzyme immunoassay is read as

Correct AnswerverifiedShow Answer

The detection of acetyl-methyl carbinol or acetoin is the basis of the _________ test.

Correct AnswerverifiedShow Answer

Which biochemical test requires the use of 10% ferric chloride to detect a positive reaction?

Correct AnswerverifiedShow Answer

A positive citrate test is due to the organism's ability to

Correct AnswerverifiedD

The proper amount and order of addition of reagent(s) for the test for the production of acetoin is

Correct AnswerverifiedShow Answer

The metabolism of carbohydrates by Pseudomonas aeruginosa is determined using

Correct AnswerverifiedShow Answer

A microbiologist sets up a set of decarboxylase tubes for identification of a glucose fermenting, gram-negative rod. After 24 hours, the tubes reveal Base control: gray Arginine: purple Lysine: yellow Ornithine: purple These tubes should be interpreted as

Correct AnswerverifiedShow Answer

A yellow ONPG test indicates production of

Correct AnswerverifiedA

Which of the following reagents are required for testing of nitrate reduction? 1) Alpha-naphthylamine 2) Sulfanilic acid 3) Zinc dust 4) Alpha-naphthol 5) 40% KOH

Correct AnswerverifiedShow Answer

The cytochrome oxidase test is used primarily for the identification of

Correct AnswerverifiedShow Answer

Which procedure is best suited for determining the HIV viral load of an individual?

Correct AnswerverifiedShow Answer

One potential disadvantage of an automated identification system is

Correct AnswerverifiedB

A non-motile, VP-positive, gram-negative rod is a possible

Correct AnswerverifiedShow Answer

In nucleic acid synthesis, which enzyme is responsible for replication of DNA?

Correct AnswerverifiedShow Answer

Phenol red carbohydrate broth should be incubated at/in ___________________ 1) -25°C. 2) 35°C. 3) ambient air. 4) CO2.

Correct AnswerverifiedShow Answer

After the addition of reagents for the nitrate test, the tube remains colorless. To determine if the organism reduced nitrates to nitrogen gas, you must now add

Correct AnswerverifiedShow Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
D

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
A

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
B

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified